Ducheyne P, Willems G, Martens M, Helsen J
J Biomed Mater Res. 1984 Mar;18(3):293-308. doi: 10.1002/jbm.820180306.
Dense and porous Ti, Ti-alloy, and stainless steel specimens were implanted in canine trabecular bone. After 6 and 12 months the specimens were retrieved together with bone tissue immediately adjacent. The trace metal content in the tissue samples was determined using neutron activation analysis, differential pulse polarography, graphite furnace atomic-absorption spectrophotometry, electron microprobe analysis, and laser microprobe analysis. The results are discussed in view of (i) the release of Ti ions, which is larger for porous than for bulk specimens, (ii) the various artifacts arising in electron microprobe (EMP) and laser microprobe mass analysis (LAMMA) determination of compositional gradients of trace metal-ion content in bone tissue, (iii) the absence of measurable quantities of V in bone tissue, and (iv) the difference in local tissue accumulation between Ti, released from Ti specimens, and Ni, released from stainless steel specimens.
将致密和多孔的钛、钛合金及不锈钢样本植入犬类松质骨中。6个月和12个月后，取出样本及紧邻的骨组织。使用中子活化分析、微分脉冲极谱法、石墨炉原子吸收分光光度法、电子微探针分析和激光微探针分析测定组织样本中的痕量金属含量。基于以下几点对结果进行了讨论：(i) 多孔样本比块状样本释放更多的钛离子；(ii) 在电子微探针（EMP）和激光微探针质量分析（LAMMA）测定骨组织中痕量金属离子含量的成分梯度时出现的各种假象；(iii) 骨组织中未检测到可测量量的钒；(iv) 钛样本释放的钛与不锈钢样本释放的镍在局部组织积累上的差异。
