Steiner M
Biochim Biophys Acta. 1983 Mar 23;729(1):17-22. doi: 10.1016/0005-2736(83)90450-9.
Platelet membranes contain colchicine-binding activity which is not due to cytoplasmic contamination. This activity could be solubilized, at least partially, by Triton X-100 and to a lesser degree by Lubrol PX. Nonidet P-40 solubilized more colchicine-binding activity than was apparent in the intact, non-detergent-treated membranes, indicating that some of the protein was available for binding in the latter. Normal membranes had a far higher binding capacity for the alkaloid vinblastine than for colchicine, both of which were measured under equilibrium conditions. Membrane tubulin was also identified by acrylamide gel electrophoresis and immunologic precipitation. The protein had characteristics similar to the cytoplasmic form but was found to be more heat resistant. These findings establish with a high degree of certainty the presence of tubulin in the platelet membrane.
血小板膜含有秋水仙碱结合活性，这并非由于细胞质污染所致。这种活性至少可以部分地被 Triton X - 100 溶解，而被 Lubrol PX 溶解的程度较小。Nonidet P - 40 溶解的秋水仙碱结合活性比完整的、未用去污剂处理的膜中明显可见的活性更多，这表明后者中一些蛋白质可用于结合。在平衡条件下测量时，正常膜对生物碱长春碱的结合能力远高于对秋水仙碱的结合能力。膜微管蛋白也通过丙烯酰胺凝胶电泳和免疫沉淀法得以鉴定。该蛋白质具有与细胞质形式相似的特征，但发现其更耐热。这些发现高度确定地证实了血小板膜中存在微管蛋白。
