Takigawa M, Imamura S
J Invest Dermatol. 1977 May;68(5):259-64. doi: 10.1111/1523-1747.ep12506651.
Anti-epidermal cell sera (AES) were obtained by immunizing rabbits with enzymatically dispersed, viable guinea-pig epidermal cells followed by absorption with guinea-pig red blood cells, spleen and thymus cells, and liver powders. Complement-mediated cytotoxicity and immunofluorescence demonstrated that AES were towards cell surface antigen specific for stratified squamous epithelia of guinea pigs, and cross reacted with the corresponding tissues of humans and monkeys. Immunofluorescence revealed that AES reacted with Hassall's corpuscles and surrounding epithelial cells of the guinea-pig thympus which seemed to share common antigens with the epidermis; AES gave no reaction with other organs. While antigens reactive with pemphigus antibodies (PA) were demonstrated by membrane immunofluorescence to be present on the epidermal cell surface, PA showed no cytotoxicity to guinea-pig and human epidermal cells. Re-treatment of isolated epidermal cells with trypsin showed that antigens ractive with PA were more susceptible to the enzyme than those reactive with AES. These findings suggest that the cell surface antigens binding AES are different from the antigens which bind PA.
抗表皮细胞血清（AES）是通过用酶分散的、活的豚鼠表皮细胞免疫兔子，然后用豚鼠红细胞、脾细胞、胸腺细胞和肝粉吸收而获得的。补体介导的细胞毒性和免疫荧光表明，AES针对豚鼠复层鳞状上皮细胞表面抗原具有特异性，并且与人类和猴子的相应组织发生交叉反应。免疫荧光显示，AES与豚鼠胸腺的哈氏小体及周围上皮细胞发生反应，这些细胞似乎与表皮共享共同抗原；AES与其他器官无反应。虽然通过膜免疫荧光证明与天疱疮抗体（PA）反应的抗原存在于表皮细胞表面，但PA对豚鼠和人类表皮细胞无细胞毒性。用胰蛋白酶对分离的表皮细胞进行再处理表明，与PA反应的抗原比与AES反应的抗原对该酶更敏感。这些发现表明，结合AES的细胞表面抗原与结合PA的抗原不同。
