Lent R
J Comp Neurol. 1984 Mar 10;223(4):548-55. doi: 10.1002/cne.902230407.
Newborn hamsters were subjected to surgical transection of their corpora callosa under hypothermic anesthesia. After completion of their development, one group of animals had their brains prepared for cyto- and myeloarchitectonic analysis. Another group had a small pellet of polyacrylamide gel containing horseradish peroxidase (HRP) implanted in different cortical loci. All were perfused with fixatives and had their brains cut into serial sections. The operated brains showed the following anatomical features: (1) The corpus callosum was partially or totally absent; (2) an abnormal longitudinal bundle was present bilaterally underneath the white matter; and (3) except for the physical displacement of some medial structures, the general architecture of the brain appeared unchanged. Analysis of HRP material revealed that (1) the longitudinal bundle contains cortical fibers, of which at least some are commissural; (2) these cortical fibers display a topographic arrangement within the bundle. Results suggest that brain anatomy of "surgical" acallosal hamsters compares closely with that observed in mice with congenital defects of the corpus callosum, a spontaneous condition which also occurs in humans.
新生仓鼠在低温麻醉下接受胼胝体手术横切。发育完成后，一组动物的大脑被用于细胞和髓鞘构筑分析。另一组在不同皮质位点植入含有辣根过氧化物酶（HRP）的聚丙烯酰胺凝胶小丸。所有动物均用固定剂灌注，大脑被切成连续切片。手术大脑呈现出以下解剖学特征：（1）胼胝体部分或完全缺失；（2）在白质下方两侧存在异常纵向束；（3）除了一些内侧结构的物理移位外，大脑的总体结构似乎未改变。对HRP物质的分析表明：（1）纵向束包含皮质纤维，其中至少一些是连合纤维；（2）这些皮质纤维在束内呈现出拓扑排列。结果表明，“手术性”无胼胝体仓鼠的脑解剖结构与先天性胼胝体缺陷小鼠中观察到的结构密切相似，这种自发情况在人类中也会出现。
