Tahara T, Kuroiwa A, Obinata M, Natori S
J Mol Biol. 1984 Mar 25;174(1):19-29. doi: 10.1016/0022-2836(84)90362-0.
Three recombinant phages containing chromosomal segments of Sarcophaga peregrina encoding storage protein were isolated. These clones were found to contain sequences hybridized with the messenger RNA of 75,000 Mr protein, a monomer of storage protein. From restriction analysis it was found that message-homologous regions are not identical, and thus are different genes with similar sequences. From the analysis of these clones it was concluded that the storage protein gene belongs to a multi-gene family.
分离出了三个含有编码嗜尸性麻蝇贮藏蛋白的染色体片段的重组噬菌体。发现这些克隆含有与75,000道尔顿蛋白(贮藏蛋白的一种单体)的信使核糖核酸杂交的序列。通过限制性分析发现,与信使核糖核酸同源的区域并不相同,因此是具有相似序列的不同基因。通过对这些克隆的分析得出结论,贮藏蛋白基因属于一个多基因家族。