Schier J F, Graczyk A, Daszyński J, Góralski S, Hełczyński L, Rudowski W
Z Exp Chir Transplant Kunstliche Organe. 1984;17(1):2-13.
In the continuing search for promoting the bio-pharmacological characteristics of an excellent oxygen carrier, the Stroma-free Haemoglobin Solution (SFHS), in the presented work, the authors were investigating the composition changes of SFHS infused to dogs. In 10 mongrel dogs a total exchange of the circulating blood was done for replacing it with SFHS. For the observing the physico-chemical changes in circulating and excreted fluids electron spectroscopy was used in view of good spectral characteristics of haemoglobin, and a high sensitivity of this method to changes taking place in the analyzed molecule. Comparing the spectral curves characterizing the solution used for exchange transfusion with the spectral curves obtained from circulating SFHS samples and haemoglobin excreted in the urine, and from the ascites, during the 6 hours course of the experiment, no essential structural changes were observed in the hemin molecule, in the visible range.
在持续探索提升优质氧载体——无基质血红蛋白溶液（SFHS）的生物药理学特性的过程中，在本研究中，作者们对输注给犬类的SFHS的成分变化进行了研究。在10只杂种犬中，进行了循环血液的全量置换，用SFHS取而代之。鉴于血红蛋白良好的光谱特性以及该方法对被分析分子中发生的变化具有高灵敏度，为观察循环和排泄液中的物理化学变化，使用了电子光谱法。在实验的6小时过程中，将表征用于换血的溶液的光谱曲线与从循环的SFHS样本、尿液和腹水中排泄的血红蛋白获得的光谱曲线进行比较，在可见范围内，未观察到血红素分子有本质的结构变化。
