Monsell G M, Shubber A H, Lloyd S, Soulsby E J
Z Parasitenkd. 1984;70(2):211-8. doi: 10.1007/BF00942224.
The in vitro transformation of lymphocytes in the presence of antigen was used to assay the response of lambs to antigens of Haemonchus contortus. Neither uninfected lambs nor lambs infected with H. contortus up to 9 weeks of age developed in vitro lymphocyte responsiveness to antigen. A slight and transient increase in reactivity was demonstrable in some of the infected lambs when these were reinfected with H. contortus at 26 weeks of age. This lack of responsiveness could be modulated through the exposure of lambs, early in neonatal life, to multiple parenteral injections of soluble third stage larval antigen of H. contortus. Thus, antigen specific responses were apparent in the lymphocytes from 21-week-old lambs if these were injected with antigen when 2 to 17 weeks of age. In particular, marked antigen specific reactivity developed in 19- or 24-week-old lambs if such lambs were injected with antigen on five occasions over the first 6 weeks of life and subsequently infected with H. contortus when 7 weeks old. This responsiveness was enhanced when the lambs were reinfected with H. contortus at 26 weeks of age.
利用淋巴细胞在抗原存在下的体外转化来测定羔羊对捻转血矛线虫抗原的反应。未感染的羔羊以及感染捻转血矛线虫达9周龄的羔羊，均未表现出体外淋巴细胞对抗原的反应性。当26周龄的部分感染羔羊再次感染捻转血矛线虫时，可证明其反应性有轻微且短暂的增加。通过在新生期早期对羔羊进行多次皮下注射捻转血矛线虫可溶性三期幼虫抗原，可调节这种无反应性。因此，如果2至17周龄的21周龄羔羊注射抗原，其淋巴细胞中会出现明显的抗原特异性反应。特别是，如果19或24周龄的羔羊在生命的前6周内接受5次抗原注射，随后在7周龄时感染捻转血矛线虫，会产生明显的抗原特异性反应。当这些羔羊在26周龄时再次感染捻转血矛线虫时，这种反应性会增强。
