Pancaldi S, Poli F, Dall'Olio G, Vannini G L
Arch Microbiol. 1984 Mar;137(3):185-7. doi: 10.1007/BF00414540.
Aspergillus niger germ tubes were exposed for 6 h to 0.15 mg/ml of Congo red, a stain which prevents chitin microfibril assembly. The most evident alterations, detected under ultraviolet light and by transmission and scanning electron microscopy, concerned the hyphal tips which burst or, most frequently, expanded into bulges. In the latter structures, new hyphal tips appeared which, after giving rise to more or less developed hyphae, were themselves converted into new bulges. Therefore, segments derived from isotropic and polarized growth alternated in the organisms exposed to the dye. An interpretation of these abnormalities is advanced based upon the assumption that the maintainance of a regular gradient of wall viscosity in the hyphal extension zone depends primarily on the capability of glycan chains to form crystalline aggregates of increasing complexity.
将黑曲霉的芽管暴露于0.15毫克/毫升的刚果红中6小时，刚果红是一种能阻止几丁质微原纤维组装的染料。在紫外光下以及通过透射电子显微镜和扫描电子显微镜检测到的最明显变化涉及菌丝尖端，这些尖端会破裂，或者最常见的是膨胀成凸起。在后者的结构中，出现了新的菌丝尖端，这些尖端在产生或多或少发育的菌丝后，自身又转化为新的凸起。因此，在接触染料的生物体中，各向同性生长和极性生长产生的片段交替出现。基于这样的假设对这些异常现象进行了解释，即菌丝延伸区壁粘度的规则梯度的维持主要取决于聚糖链形成日益复杂的晶体聚集体的能力。
