Wilce P A, Leijten L, Martin L
Biochem J. 1984 Mar 15;218(3):849-55. doi: 10.1042/bj2180849.
The characteristics of 3-hydroxy-3-methylglutaryl-CoA reductase from mouse uterine epithelial cells were studied. Preliminary experiments showed that enzyme activity was stimulated approx. 10-fold 18h after administration of 100ng of oestradiol-17 beta. This activity was associated with all particulate fractions of the uterine luminal cell. The Km for D-3-hydroxy-3-methylglutaryl-CoA was 5.54 +/- 1.12 microM. The detailed time-course of oestrogen stimulation showed two peaks of activity, 9 and 15h after hormone treatment. The DNA content of the epithelial cells doubled between 6 and 12h after hormone treatment, whereas the protein content increased linearly over the 18h period. The peak of enzyme activity at 9h is associated with early S phase of the epithelial cells; the peak at 15h may be associated with a second S phase or with mitosis. Pretreatment with progesterone for 3 days before injection of oestradiol-17 beta (a treatment which inhibits uterine epithelial DNA synthesis) reduced the oestrogenic stimulation of enzyme activity by 63%; progesterone treatment alone did not stimulate enzyme activity. These data suggest that uterine epithelial 3-hydroxy-3-methylglutaryl-CoA reductase may play an important role in the cell cycle in this tissue.
对小鼠子宫上皮细胞中3-羟基-3-甲基戊二酰辅酶A还原酶的特性进行了研究。初步实验表明，给予100 ng的17β-雌二醇后18小时，酶活性大约被刺激了10倍。这种活性与子宫腔细胞的所有颗粒组分相关。D-3-羟基-3-甲基戊二酰辅酶A的米氏常数为5.54±1.12微摩尔。雌激素刺激的详细时间进程显示，在激素处理后9小时和15小时出现两个活性峰值。激素处理后6至12小时，上皮细胞的DNA含量增加了一倍，而蛋白质含量在18小时内呈线性增加。9小时时的酶活性峰值与上皮细胞的早期S期相关；15小时时的峰值可能与第二个S期或有丝分裂相关。在注射17β-雌二醇前用孕酮预处理3天（这种处理可抑制子宫上皮DNA合成），使雌激素对酶活性的刺激降低了63%；单独使用孕酮处理不会刺激酶活性。这些数据表明，子宫上皮3-羟基-3-甲基戊二酰辅酶A还原酶可能在该组织的细胞周期中起重要作用。
