Capel I D, Dorrell H M
Biochem J. 1984 Apr 1;219(1):41-9. doi: 10.1042/bj2190041.
The concentration of lipoperoxides (estimated as thiobarbituric acid-reactive material) and some components of the antioxidant defence system have been compared in various tissues of lean and congenitally obese mice. NADPH-stimulated lipoperoxide generation in vitro was significantly higher in microsomes (microsomal fractions) prepared from obese hepatic tissue than lean. Plasma, liver and brain lipoperoxide concentration was significantly higher in obese mice. In blood derived from obese mice the concentration of non-enzymic antioxidants including caeruloplasmin and vitamin A was higher, but hepatic retinol concentration was lower in these animals. In all the tissues assayed the glutathione peroxidase activity against H2O2 was less than its activity against cumene hydroperoxide. Assayed with either substrate, glutathione peroxidase activity was significantly higher in the brain and blood of obese mice than their lean counterparts. Conversely, liver glutathione peroxidase was decreased in obese animals, representing 43% of the activity of the lean-mouse liver enzyme against H2O2 and 81% of the cumene hydroperoxide-reducing activity. The liver of obese mice had significantly less, and the kidneys more, oxidized glutathione than the corresponding tissues of lean mice. Further investigations on hepatic tissue indicated that glutathione reductase activity was lower in the obese animals, but there was no significant difference between glucose-6-phosphate dehydrogenase activity in obese and lean mice.
已对瘦小鼠和先天性肥胖小鼠的各种组织中脂过氧化物（以硫代巴比妥酸反应性物质估算）的浓度以及抗氧化防御系统的某些成分进行了比较。在体外，由肥胖肝脏组织制备的微粒体（微粒体部分）中NADPH刺激的脂过氧化物生成显著高于瘦小鼠。肥胖小鼠的血浆、肝脏和脑脂过氧化物浓度显著更高。在肥胖小鼠的血液中，包括铜蓝蛋白和维生素A在内的非酶抗氧化剂浓度更高，但这些动物的肝脏视黄醇浓度更低。在所有检测的组织中，谷胱甘肽过氧化物酶对过氧化氢的活性低于其对氢过氧化异丙苯的活性。无论使用哪种底物进行检测，肥胖小鼠脑和血液中的谷胱甘肽过氧化物酶活性均显著高于瘦小鼠。相反，肥胖动物肝脏中的谷胱甘肽过氧化物酶活性降低，相对于瘦小鼠肝脏酶对过氧化氢的活性而言为43%，对氢过氧化异丙苯还原活性而言为81%。与瘦小鼠的相应组织相比，肥胖小鼠的肝脏中氧化型谷胱甘肽显著减少，而肾脏中则更多。对肝脏组织的进一步研究表明，肥胖动物的谷胱甘肽还原酶活性较低，但肥胖小鼠和瘦小鼠的葡萄糖-6-磷酸脱氢酶活性之间没有显著差异。
