ten Cate H, Lamping R J, Henny C P, Prins A, ten Cate J W
Clin Chem. 1984 Jun;30(6):860-4.
Using the chromogenic substrate S-2222, we have optimized and automated an amidolytic assay for heparin. The assay is based on the detection of anti-Xa activity generated by heparin in plasma. The method is reproducible (intra- and interassay CVs of 2.4 and 3.3%, respectively) and reliable in antithrombin III-deficient plasma. Results of this assay, obtained for plasma samples from patients and volunteers treated with heparin, correlate well (r = 0.899) with those of the test for activated partial thromboplastin time. Upon administration of a low-Mr heparinoid (Org 10172) and heparin fragment ( Kabi 2165), however, the activated partial thromboplastin time failed to detect anticoagulant activity, whereas the chromogenic heparin assay revealed anti-Xa activity. This automated amidolytic assay for heparin is therefore suitable not only for monitoring standard therapy with heparin but also for measuring the activity of recently developed heparin fractions.
我们使用发色底物S-2222对肝素的酰胺水解测定法进行了优化和自动化处理。该测定法基于检测血浆中肝素产生的抗Xa活性。该方法具有可重复性（批内和批间变异系数分别为2.4%和3.3%），并且在抗凝血酶III缺乏的血浆中可靠。对接受肝素治疗的患者和志愿者的血浆样本进行该测定法的结果，与活化部分凝血活酶时间检测结果具有良好的相关性（r = 0.899）。然而，给予低分子量类肝素（Org 10172）和肝素片段（Kabi 2165）后，活化部分凝血活酶时间未能检测到抗凝活性，而发色肝素测定法显示出抗Xa活性。因此，这种肝素的自动化酰胺水解测定法不仅适用于监测肝素的标准治疗，还适用于测量最近开发的肝素组分的活性。
