van Putten J, van de Ruit M, Beunis M, Hemker H C
Haemostasis. 1984;14(2):205-10. doi: 10.1159/000215057.
The applicability was investigated of automated spectrophotometric heparin assays and three clotting assays for determination of two low molecular weight (LMW) heparin fractions: Org 10172 and DxN10 and two infractionated commercially available heparins. The relative activity of the two commercially available heparins was similar in the anti-Xa assay, in the anti-IIa assay and in 3 clotting assays. The LMW heparins showed markedly different relative activity in all 5 assays. The activities of those heparin preparations relative to the standard heparin were compared in the 5 assays, but standardization against a standard heparin preparation appeared impossible. Methods of heparin determination can be used to monitor treatment with a heparin preparation only if the same preparation is used as a reference substance.
研究了自动分光光度法肝素测定法和三种凝血测定法在测定两种低分子量（LMW）肝素制剂（Org 10172和DxN10）以及两种市售分级肝素中的适用性。两种市售肝素在抗Xa测定、抗IIa测定和三种凝血测定中的相对活性相似。低分子量肝素在所有五种测定中显示出明显不同的相对活性。在这五种测定中比较了这些肝素制剂相对于标准肝素的活性，但以标准肝素制剂进行标准化似乎是不可能的。只有当使用相同的制剂作为参考物质时，肝素测定方法才能用于监测肝素制剂的治疗。
