Rama S, Chandrakasan G
Connect Tissue Res. 1984;12(2):111-8. doi: 10.3109/03008208408992776.
It is known that cartilage collagen in higher vertebrates conforms to Type II collagen but very little is known of the nature of shark cartilage. This study was undertaken to determine the differences, if any, between shark cartilage collagen and that of higher vertebrates. Collagen was obtained from shark cartilage by pepsin solubilization and characterized by amino acid analysis and determination of chain composition by SDS-polyacrylamide gel electrophoresis and CM-cellulose chromatography. Results indicated the presence not only of Type II collagen but also of Type I collagen. Type I collagen accounted for about one third of the total collagen content of shark cartilage.
已知高等脊椎动物的软骨胶原蛋白符合II型胶原蛋白，但对于鲨鱼软骨的性质却知之甚少。本研究旨在确定鲨鱼软骨胶原蛋白与高等脊椎动物的软骨胶原蛋白之间是否存在差异。通过胃蛋白酶溶解从鲨鱼软骨中获取胶原蛋白，并通过氨基酸分析、SDS-聚丙烯酰胺凝胶电泳和CM-纤维素色谱法测定链组成对其进行表征。结果表明，鲨鱼软骨中不仅存在II型胶原蛋白，还存在I型胶原蛋白。I型胶原蛋白约占鲨鱼软骨总胶原蛋白含量的三分之一。
