Fernandez H L, Seiter T C
FEBS Lett. 1984 May 7;170(1):147-51. doi: 10.1016/0014-5793(84)81388-5.
This study describes the changes which occur in intra- and extracellular asymmetric acetylcholinesterase (AChE) forms in rat gracilis muscle during postnatal development. Initially (day 7) these forms (12.5 S and 16 S AChE) were evenly distributed along the muscle and only present intracellularly. With advancing age (days 7-28) they gradually became concentrated in endplate (vs non-endplate) muscle regions where a certain proportion of them was subsequently externalized. In contrast, no externalization was observed in the non-endplate regions. Our results support the view that AChE asymmetric forms are assembled within the muscle cell prior to their deposition on the extracellular synaptic compartment.
本研究描述了大鼠股薄肌在出生后发育过程中细胞内和细胞外不对称乙酰胆碱酯酶（AChE）形式的变化。最初（第7天），这些形式（12.5S和16S AChE）沿肌肉均匀分布，且仅存在于细胞内。随着年龄增长（第7 - 28天），它们逐渐集中在终板（与非终板相对）肌肉区域，随后其中一定比例的酶被外化。相比之下，在非终板区域未观察到外化现象。我们的结果支持这样一种观点，即AChE不对称形式在沉积到细胞外突触区室之前是在肌肉细胞内组装的。
