Fernandez H L, Inestrosa N C, Stiles J R
Neurochem Res. 1984 Sep;9(9):1211-30. doi: 10.1007/BF00973035.
The characterization of individual acetylcholinesterase (AChE) molecular form subcellular pools in adult mammalian skeletal muscle is a critical point when considering such questions as the origin, assembly, and neurotrophic regulation of these molecules. By correlating the results of differential extraction, in vitro collagenase digestion, and in situ pharmacologic probes of AChE molecular forms in endplate regions of adult rat anterior gracilis muscle, we have shown that: 1) 4.0S (G1) and 6.0S (G2) AChE are predominantly membrane-bound and intracellular; if an extracellular and/or soluble fraction of these forms exists, it cannot be adequately resolved by our methods; 2) 9-11S (globular) AChE activity is distributed between internal and external pools, as well as membrane-associated and soluble fractions; 3) 16.0S (A12) AChE is not an integral membrane protein and exists both intracellularly (25-30%) and extracellularly (70-75%).
在考虑诸如成年哺乳动物骨骼肌中这些分子的起源、组装和神经营养调节等问题时，对成年哺乳动物骨骼肌中单个乙酰胆碱酯酶（AChE）分子形式亚细胞池的表征是一个关键点。通过将成年大鼠股薄肌终板区域AChE分子形式的差异提取、体外胶原酶消化和原位药理学探针的结果相关联，我们发现：1）4.0S（G1）和6.0S（G2）AChE主要与膜结合且存在于细胞内；如果这些形式存在细胞外和/或可溶性部分，我们的方法无法充分解析；2）9 - 11S（球状）AChE活性分布于内部和外部池以及膜相关和可溶性部分；3）16.0S（A12）AChE不是完整的膜蛋白，既存在于细胞内（25 - 30%）也存在于细胞外（70 - 75%）。
