Touraine J L, Touraine F, Hadden J W, Hadden E M, Good R A
Int Arch Allergy Appl Immunol. 1976;52(1-4):105-17. doi: 10.1159/000231672.
The study of human peripheral blood currently permits enumeration of circulating B and T lymphocytes as well as the analysis of functional responses in vitro following stimulation with mitogens, antigens or allogeneic cells. In the present experiments, subsets of these major lymphocyte populations were analyzed by dissecting in vitro responses using an ablative technique. After an initial culture period of lymphocytes with a mitogen, the proliferating cells were inactivated with 5-bromodeoxyuridine and light, then the capacity of the remaining lymphocytes to respond to the same or a different mitogenic influence was tested. Responsiveness to a different stimulant in the presence of no further response to the first stimulant was taken as evidence for a different responding cell population. A large subset of peripheral blood lymphocytes was responsive to both phytohemagglutinin (PHA) and concanavalin A (Con A); ablation of the cells responsive to one left little or no cells responsive to the other. Pokeweed mitogen (PWM) stimulated a portion of the PHA-Con A-responsive subset and an approximately equal subset unresponsive to PHA or Con A. Other evidence indicates that with each of these mitogens (especially with PHA and Con A in a soluble form), most of the proliferative response of peripheral blood B lymphocytes is indirectly triggered and is dependent on T cell stimulation. The population of PHA-Con A-responsive cells is, therefore, interpreted to represent a major T cell subset plus recruited cells; the PWM-responsive population would include a T cell subset having also PHA and Con A responsiveness, and another subset of T (or perhaps B) cells. The mitogen-sensitive population showed no overlap with cells responsive to allogeneic stimulation in mixed leukocyte culture. Ablation of the mitogen-responsive cells potentiated the mixed leukocyte reaction, suggesting that a suppressive influence was removed with the inactivation of the mitogen-responsive cells. It appears, therefore, that distinct subsets of T lymphocytes differentially responsive to PHA-Con A, to PWM and to allogeneic stimulation are present in the human peripheral blood.
目前，对人类外周血的研究能够对外周血中循环的B淋巴细胞和T淋巴细胞进行计数，还能分析在有丝分裂原、抗原或同种异体细胞刺激后体外的功能反应。在本实验中，通过使用一种消除技术剖析体外反应，对这些主要淋巴细胞群体的亚群进行了分析。在用有丝分裂原对淋巴细胞进行初始培养期后，用5-溴脱氧尿苷和光照使增殖细胞失活，然后测试剩余淋巴细胞对相同或不同有丝分裂原刺激的反应能力。在对第一种刺激物不再有反应的情况下对不同刺激物有反应，被视为存在不同反应细胞群体的证据。外周血淋巴细胞的一大亚群对植物血凝素（PHA）和刀豆球蛋白A（Con A）均有反应；消除对其中一种有反应的细胞后，对另一种有反应的细胞所剩无几或几乎没有。美洲商陆有丝分裂原（PWM）刺激了一部分对PHA-Con A有反应的亚群以及一个对PHA或Con A无反应的大致相等的亚群。其他证据表明，对于这些有丝分裂原中的每一种（尤其是可溶性形式的PHA和Con A），外周血B淋巴细胞的大多数增殖反应是间接触发的，并且依赖于T细胞刺激。因此，对PHA-Con A有反应的细胞群体被解释为代表一个主要的T细胞亚群加上募集的细胞；对PWM有反应的群体将包括一个也对PHA和Con A有反应的T细胞亚群，以及另一个T（或可能是B）细胞亚群。有丝分裂原敏感群体与混合淋巴细胞培养中对同种异体刺激有反应的细胞没有重叠。消除有丝分裂原反应性细胞增强了混合淋巴细胞反应，这表明随着有丝分裂原反应性细胞的失活，一种抑制性影响被消除了。因此，似乎人类外周血中存在对PHA-Con A、对PWM以及对同种异体刺激有不同反应的不同T淋巴细胞亚群。
