Kelly M K, Thong Y H
Immunol Lett. 1984;7(6):309-13. doi: 10.1016/0165-2478(84)90086-5.
We studied the rate of decay of granulocyte adherence following isolation from human blood using the nylon-fibre microcolumn technique recently developed in our laboratory. The decay in adherence was found to be evident by 6 h, pronounced by 24 h, and very substantial by 48 h. This decay in adherence can be reversed by incubation at 4 degrees C instead of 37 degrees C. It can also be retarded by incubation with both superoxide dismutase and 2,3-dihydroxybenzoic acid. The data suggests that peroxidation of the granulocyte membrane is a possible mechanism for the decay in granulocyte adherence. These results provide further insight into biological aspects of granulocyte activity, and have implications for the laboratory evaluation of granulocyte function.
我们使用本实验室最近开发的尼龙纤维微柱技术，研究了从人血中分离后粒细胞黏附的衰减速率。发现黏附衰减在6小时时明显，24小时时显著，48小时时非常明显。这种黏附衰减可通过在4℃而非37℃下孵育来逆转。同时与超氧化物歧化酶和2,3 - 二羟基苯甲酸一起孵育也可使其减缓。数据表明粒细胞膜的过氧化是粒细胞黏附衰减的一种可能机制。这些结果为粒细胞活性的生物学方面提供了进一步的见解，并对粒细胞功能的实验室评估具有启示意义。
