MacGregor R R, Macarak E J, Kefalides N A
J Clin Invest. 1978 Mar;61(3):697-702. doi: 10.1172/JCI108981.
Adherence of granulocytes to tissue culture monolayers of endothelium averaged 26.2 +/- 1.3% SEM, which was similar to their adherence on 50-mg nylon fiber columns (27.7 +/- 3.6%). In contrast, adherence to epithelial cells, fibroblasts, kidney cells, and plastic Petri dishes without monolayers was only 12.4, 9.9, 11.1, and 4.3%, respectively. Cyclic nucleotides and adherence-modifying plasma factors induced changes of adherence to endothelium similar to those in nylon fiber columns. Adherence of granulocytes in whole blood was the same as for purified granulocytes in Hank's balanced salt solution. Exposure of endothelial monolayers to 0.18% trypsin for 10 min reduced subsequent granulocyte adherence to 25.2% of control values. Incubation of trypsin-treated monolayers with nutrient medium for 4 h did not improve adherence, but values returned to normal or above by 24 h, with or without serum proteins present in the nutrient medium. The similarity of granulocyte adherence to nylon fiber and to endothelial monolayers in vitro suggests that results with the nylon fiber assay reflect in vivo granulocyte-endothelium interaction. Furthermore, the endothelial monolayer offers a new model for studying this cell-cell relationship in vitro.
粒细胞与内皮细胞组织培养单层的黏附率平均为26.2±1.3%（标准误），这与它们在50毫克尼龙纤维柱上的黏附率（27.7±3.6%）相似。相比之下，粒细胞与上皮细胞、成纤维细胞、肾细胞以及没有单层细胞的塑料培养皿的黏附率分别仅为12.4%、9.9%、11.1%和4.3%。环核苷酸和能改变黏附的血浆因子引起的与内皮细胞的黏附变化，与在尼龙纤维柱上的情况相似。全血中粒细胞的黏附情况与汉克平衡盐溶液中纯化粒细胞的黏附情况相同。将内皮细胞单层暴露于0.18%的胰蛋白酶中10分钟，会使随后粒细胞的黏附率降至对照值的25.2%。用营养培养基孵育经胰蛋白酶处理的单层细胞4小时，黏附率并未提高，但无论营养培养基中是否存在血清蛋白，24小时后黏附率会恢复到正常或高于正常水平。粒细胞在体外与尼龙纤维和内皮细胞单层的黏附情况相似，这表明尼龙纤维检测法的结果反映了体内粒细胞与内皮细胞的相互作用。此外，内皮细胞单层为体外研究这种细胞间关系提供了一个新模型。
