Arneson R M, Aloyo V J, Germain G S, Chenevey J E
Lipids. 1978 Jun;13(6):383-90. doi: 10.1007/BF02533706.
Cultured mouse neuroblastoma cells exhibit a striking increase in antioxidative capacity during the transition from logarithmically dividing cells to nondividing, neurite-bearing cells. Two physically separable phenomena are involved: (a) the membrane pellet of neurite-bearing cells is highly resistant to lipid peroxidation, and (b) the postmicrosomal supernatant of these cells inhibits peroxidation in rat liver mitochondria and other biological membranes. A precise, single-phase assay has been developed for assessing antioxidant levels in lipid extracts. By means of this assay, the increase in membrane resistance to lipid peroxidation has been correlated with a threefold increase in the antioxidant activity of the neuroblastoma neutral lipid fraction. This finding implies that generations of a neutral lipid antioxidant (or antioxidants) is involved in the profound increase in antioxidative capacity which occurs in differentiating neuroblastoma cells.
培养的小鼠神经母细胞瘤细胞在从对数生长期的分裂细胞转变为不分裂的、带有神经突的细胞的过程中，抗氧化能力显著增强。这涉及两个在物理上可分离的现象：(a) 带有神经突的细胞的膜沉淀对脂质过氧化具有高度抗性，(b) 这些细胞的微粒体后上清液可抑制大鼠肝线粒体和其他生物膜中的过氧化反应。已开发出一种精确的单相测定法来评估脂质提取物中的抗氧化剂水平。通过该测定法，膜对脂质过氧化抗性的增加与神经母细胞瘤中性脂质部分抗氧化活性增加三倍相关。这一发现表明，一种中性脂质抗氧化剂（或多种抗氧化剂）的产生与分化中的神经母细胞瘤细胞抗氧化能力的显著增强有关。
