Leberer E, Pette D
Histochemistry. 1984;80(3):295-8. doi: 10.1007/BF00495780.
Lactate dehydrogenase (LDH) isozyme patterns were analyzed by polyacrylamide (PAA) slab gel electrophoresis in extracts prepared from various rabbit skeletal muscles of defined fibre composition and by PAA microelectrophoresis of microdissected, histochemically typed single muscle fibres. The results obtained by electrophoresis of whole muscle extracts generally agreed with the data obtained from single fibre electrophoresis, i.e. the LDH isozyme pattern corresponded to that of the predominant fibre type. Type I fibres from soleus and semitendinosus muscles were characterized by a unique pattern of all 5 LDH isozymes with a predominance of LDH-1, 2 and 3. The major fraction (80%) of the type II fibres from extensor digitorum longus and tibialis anterior muscles contained only LDH-5 (M4). About 20% of the type II fibres contained in addition to LDH-5 small amounts of LDH-4 and LDH-3. The fraction of fibres containing LDH-5, LDH-4, and LDH-3 was similar (ca. 20%) in the histochemically defined IIA and IIB subpopulations. In view of the fact that the major fractions of rabbit IIB fibres display low and of IIA fibres high aerobic oxidative capacities ( Reichmann and Pette 1982), these data indicate that the expression of the H-subunit of LDH is not correlated with the aerobic-oxidative capacity of the fibre. It also appears not to be correlated with the presence of different myosin isoforms in IIA and IIB fibres.
通过聚丙烯酰胺（PAA）平板凝胶电泳，对从具有明确纤维组成的各种兔骨骼肌制备的提取物进行乳酸脱氢酶（LDH）同工酶模式分析，并通过对显微解剖的、经组织化学分型的单根肌纤维进行PAA微电泳分析。对全肌肉提取物进行电泳得到的结果与从单根纤维电泳获得的数据总体一致，即LDH同工酶模式与主要纤维类型的模式相对应。比目鱼肌和半腱肌的I型纤维的特征是所有5种LDH同工酶具有独特的模式，其中LDH-1、2和3占优势。来自趾长伸肌和胫骨前肌的II型纤维的主要部分（80%）仅含有LDH-5（M4）。约20%的II型纤维除含有LDH-5外，还含有少量的LDH-4和LDH-3。在组织化学定义的IIA和IIB亚群中，含有LDH-5、LDH-4和LDH-3的纤维比例相似（约20%）。鉴于兔IIB纤维的主要部分显示出低有氧氧化能力，而IIA纤维显示出高有氧氧化能力（Reichmann和Pette，1982），这些数据表明LDH的H亚基的表达与纤维的有氧氧化能力无关。它似乎也与IIA和IIB纤维中不同肌球蛋白同工型的存在无关。
