Reichmann H, Pette D
Histochemistry. 1984;80(5):429-33. doi: 10.1007/BF00495430.
Quantitative microphotometric measurements of two mitochondrial flavoproteins, glycerolphosphate oxidase (GP-OX) and succinate dehydrogenase (SDH), were performed on serial sections of mouse and rabbit tibialis anterior (TA) muscles in order to study the distribution of these two enzymes and their activity ratios in IIA and IIB fibres. The measurements showed a large scatter of the two enzyme activities in these two myosin-based fibre types. In rabbit TA, IIA and IIB fibres have similar GP-OX activities, whereas generally IIA fibres have higher SDH activities than IIB fibres. An inverse distribution of the two enzymes exists in mouse muscle. Generally, IIA fibres of mouse TA display low SDH and IIB fibres high SDH activities. The mean activity of GP-OX is slightly higher in IIA than in IIB fibres of mouse TA. Since measurements of both enzymes were taken in the same fibres, the ratio of their activities in each fibre could be evaluated. The SDH/GP-OX activity ratios vary significantly between the two fibre populations both in rabbit and in mouse. The ratio is high in IIA and low in IIB fibres of rabbit TA, whereas it is low in IIA and high in IIB fibres of mouse TA.
为了研究甘油磷酸氧化酶（GP - OX）和琥珀酸脱氢酶（SDH）这两种线粒体黄素蛋白在小鼠和家兔胫骨前肌（TA）IIA和IIB型纤维中的分布及其活性比率，对其连续切片进行了定量显微光度测量。测量结果显示，在这两种基于肌球蛋白的纤维类型中，这两种酶的活性存在很大差异。在家兔TA中，IIA和IIB型纤维的GP - OX活性相似，而通常IIA纤维的SDH活性高于IIB纤维。在小鼠肌肉中，这两种酶呈反向分布。一般来说，小鼠TA的IIA纤维显示低SDH活性，而IIB纤维显示高SDH活性。小鼠TA的IIA纤维中GP - OX的平均活性略高于IIB纤维。由于在同一纤维中对两种酶都进行了测量，因此可以评估每根纤维中它们的活性比率。家兔和小鼠的两种纤维群体之间，SDH/GP - OX活性比率存在显著差异。家兔TA的IIA纤维中该比率高，IIB纤维中该比率低，而小鼠TA的IIA纤维中该比率低，IIB纤维中该比率高。
