Fricker C R
J Appl Bacteriol. 1984 Apr;56(2):305-9. doi: 10.1111/j.1365-2672.1984.tb01351.x.
The efficiency of Rappaport's broth ( RB10 ) and Rappaport's broth containing novobiocin ( NRB10 ) were compared for the isolation of salmonellas from polluted water, both as direct enrichment media and after pre-enrichment in buffered peptone water. Ninety samples were examined and 41 were found to contain salmonellas by at least one of the procedures used. Direct inoculation of the sample into RB10 resulted in the recovery of salmonellas from only 29.3% of the samples found to be positive. The use of NRB10 as a direct enrichment medium increased the percentage recovery to 78.0% of the total positive samples. Pre-enrichment in buffered peptone water allowed the recovery of salmonellas from a total of 41 samples whereas direct enrichment recovered them from only 32. No significant difference was demonstrated in the efficiencies of RB10 and NRB10 in recovering salmonellas after pre-enrichment in buffered peptone water. Three selective agars were used: no significant difference in their ability to recover salmonellae was demonstrated.
比较了Rappaport肉汤（RB10）和含新生霉素的Rappaport肉汤（NRB10）从污染水中分离沙门氏菌的效率，二者均作为直接增菌培养基以及在缓冲蛋白胨水中预增菌后使用。检测了90个样本，通过至少一种所用方法发现41个样本含有沙门氏菌。将样本直接接种到RB10中，仅从29.3%被发现为阳性的样本中检出了沙门氏菌。使用NRB10作为直接增菌培养基，阳性样本的回收率提高到了78.0%。在缓冲蛋白胨水中预增菌可从总共41个样本中检出沙门氏菌，而直接增菌仅从32个样本中检出。在缓冲蛋白胨水中预增菌后，RB10和NRB10在回收沙门氏菌的效率上未显示出显著差异。使用了三种选择性琼脂：它们在回收沙门氏菌的能力上未显示出显著差异。
