Mecham R P, Griffin G L, Madaras J G, Senior R M
J Cell Biol. 1984 May;98(5):1813-6. doi: 10.1083/jcb.98.5.1813.
We studied chemotaxis to elastin peptides by bovine ligamentum nuchae fibroblasts to determine whether there is a developmental association between chemotactic responsiveness to elastin and expression of the elastin phenotype. Undifferentiated ligament cells demonstrate chemotactic responsiveness to platelet-derived growth factor and fibronectin, known chemoattractants for fibroblasts, but do not show chemotaxis to elastin peptides. After matrix-induced differentiation, however, young cells display a positive chemotactic response to elastin that persists even after the cells are removed from the matrix substratum. Matrix-induced chemotaxis to elastin could be inhibited selectively by incorporation of bromodeoxyuridine into DNA of undifferentiated cells before (but not after) contact with inducing matrix. These results show that the appearance of chemotaxis to elastin peptides parallels the onset of elastin synthesis and suggests that the acquisition of chemotactic responsiveness to elastin and expression of the elastin phenotype are affected by the same inducing elements or processes and may be closely coupled in development.
我们研究了牛项韧带成纤维细胞对弹性蛋白肽的趋化作用，以确定对弹性蛋白的趋化反应性与弹性蛋白表型的表达之间是否存在发育关联。未分化的韧带细胞对血小板衍生生长因子和纤连蛋白（已知的成纤维细胞趋化因子）表现出趋化反应性，但对弹性蛋白肽不表现出趋化作用。然而，在基质诱导分化后，年轻细胞对弹性蛋白表现出阳性趋化反应，即使在细胞从基质底物中移除后这种反应仍持续存在。在未分化细胞与诱导基质接触之前（而非之后），通过将溴脱氧尿苷掺入DNA中，可以选择性地抑制基质诱导的对弹性蛋白的趋化作用。这些结果表明，对弹性蛋白肽趋化作用的出现与弹性蛋白合成的开始平行，并表明对弹性蛋白趋化反应性的获得和弹性蛋白表型的表达受相同的诱导因素或过程影响，并且在发育过程中可能紧密相关。
