胚胎癌细胞分化过程中功能性胰岛素受体的出现。
Appearance of functional insulin receptors during the differentiation of embryonal carcinoma cells.
作者信息
Heath J, Bell S, Rees A R
出版信息
J Cell Biol. 1981 Oct;91(1):293-7. doi: 10.1083/jcb.91.1.293.
Abstract
The insulin-receptor binding activity and insulin-stimulated growth response of PC13 clone 5 cells were investigated for both the embryo carcinoma (EC) and retinoic acid-induced differentiated derivatives of this cell line. Whereas the EC cell was found to have very few, if any, receptors and showed no demonstrable dependence on insulin for growth, the differentiated derivative cell expressed a large number of insulin receptors and, when challenged with the hormone, showed stimulation of both DNA synthesis and cell division. The same data were obtained for five independent PC13 clones. These results, coupled with previous observations, lend weight to the suggestion that the appearance of specific receptors for growth regulatory substances may be a manifestation of a general change in growth-regulatory mechanisms accompanying EC cell differentiation and loss of malignancy.
摘要
对该细胞系的胚胎癌（EC）细胞以及视黄酸诱导分化的衍生物PC13克隆5细胞的胰岛素受体结合活性和胰岛素刺激的生长反应进行了研究。虽然发现EC细胞即使有受体也非常少，并且未显示出对胰岛素生长的明显依赖性，但分化的衍生物细胞表达了大量胰岛素受体，并且在受到该激素刺激时，显示出DNA合成和细胞分裂均受到刺激。对五个独立的PC13克隆获得了相同的数据。这些结果，再加上先前的观察结果，支持了这样一种观点，即生长调节物质特异性受体的出现可能是伴随EC细胞分化和恶性丧失的生长调节机制普遍变化的一种表现。
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