Takasaki S, Emling F, Leive L
J Cell Biol. 1984 Jun;98(6):2198-203. doi: 10.1083/jcb.98.6.2198.
Variants that lack the ability to ingest latex beads were isolated from the mouse macrophagelike cell line J774. Carboxylated latex beads were derivatized with polylysine and then daunomycin by a carbodiimide method. Cells that ingested such beads were killed; variants that survived were isolated. Variants were detected at very low frequency and only after nitrosoguanidine mutagenesis. Of 11 independent isolates, 10 showed a lowered rate of uptake of polystyrene beads (without daunomycin). All of these proved normal in rate and extent of Fc-mediated phagocytosis. There was essentially no change in sensitivity to free daunomycin in the variants compared to the parent. These results support the previous hypothesis that there are differences in the metabolic routes of receptor-mediated and nonspecific phagocytosis.
从小鼠巨噬细胞样细胞系J774中分离出缺乏摄取乳胶珠能力的变体。用聚赖氨酸对羧化乳胶珠进行衍生化，然后通过碳二亚胺法用柔红霉素进行衍生化。摄取此类珠子的细胞被杀死；存活的变体被分离出来。变体在极低频率下被检测到，且仅在亚硝基胍诱变后才出现。在11个独立分离株中，10个显示聚苯乙烯珠（无柔红霉素）的摄取率降低。所有这些在Fc介导的吞噬作用的速率和程度方面均表现正常。与亲本相比，变体对游离柔红霉素的敏感性基本没有变化。这些结果支持了先前的假设，即受体介导的吞噬作用和非特异性吞噬作用的代谢途径存在差异。
