Mattoo A K, St John J B, Wergin W P
J Cell Biochem. 1984;24(2):163-75. doi: 10.1002/jcb.240240207.
Cultivation of Spirodela oligorrhiza ( Kurtz ) Hegelm on a sublethal dose of atrazine results in a higher linolenic to linoleic acid ratio in the thylakoid membrane lipids, less starch, more osmiophilic globules, and a reduced stroma lamellar system. Also, the grana become randomly oriented and contain more numerous and elongated lamellae. These alterations in the lipid composition and ultrastructure of the chloroplast resemble those previously observed in triazine-resistant weed biotypes and in chloroplasts developed under low light. Thylakoid membranes from atrazine-adapted plants revealed an additional high-affinity binding constant for [14C]-diuron but the number of diuron binding sites actually decreased by 20 times compared to controls. The 32,000-dalton membrane protein of the chloroplast is synthesized actively, but its breakdown appears decreased compared to control plants. The adaptive reorganization of thylakoid components may be a compensatory mechanism for maintenance of a functional interaction of the proteins and lipids of the photosystem II complex.
用亚致死剂量的莠去津培养少根紫萍（库尔茨）黑格尔姆，会导致类囊体膜脂中亚麻酸与亚油酸的比例升高，淀粉减少，嗜锇小球增多，基质片层系统减少。此外，基粒变得随机取向，包含更多且更长的片层。叶绿体脂质组成和超微结构的这些变化类似于先前在抗三嗪杂草生物型和弱光下发育的叶绿体中观察到的变化。来自适应莠去津植物的类囊体膜显示出对[14C] - 敌草隆的额外高亲和力结合常数，但与对照相比，敌草隆结合位点的数量实际上减少了20倍。叶绿体的32,000道尔顿膜蛋白被积极合成，但其降解似乎比对照植物减少。类囊体成分的适应性重组可能是维持光系统II复合物蛋白质和脂质功能相互作用的一种补偿机制。
