Molchanov M I, Mezentsev V V
Biokhimiia. 1978 Aug;43(8):1429-37.
The role of 14C-aminoacyl-tRNAs in the formation of aminoacyl phosphatidyl glycerols in isolated chloroplasts of haricot bean leaves was studied. The formation of 14C-aminoacyl-tRNAs was more intensive in the case when 14C-aminoacyl phosphatidyl glycerols were the source of amino acids. On incubation of lamellae with 14C-aminoacyl phosphatidyl glycerols, 14C-amino acids proved to be incorporated intensively in protein of the lamellae. Membrane-bound chloroplast ribosome-like particles were observed on the outermost thylakoid membranes of the grana stacks as well as on the stroma thylakoids. It is concluded that aminoacyl phosphatidyl glycerols play an important role in lateral transport of amino acids within the chloroplasts lamellar system.
研究了14C-氨酰基转移核糖核酸(14C-氨基酰-tRNAs)在菜豆叶片离体叶绿体中氨酰基磷脂酰甘油形成过程中的作用。当14C-氨酰基磷脂酰甘油作为氨基酸来源时,14C-氨基酰-tRNAs的形成更为强烈。用14C-氨酰基磷脂酰甘油孵育类囊体时,14C-氨基酸被大量掺入类囊体蛋白中。在基粒垛叠最外层的类囊体膜以及基质类囊体上观察到了膜结合的叶绿体核糖体样颗粒。得出的结论是,氨酰基磷脂酰甘油在叶绿体类囊体系统内氨基酸的侧向运输中起重要作用。