Sato M, Paschen W, Pawlik G, Heiss W D
J Cereb Blood Flow Metab. 1984 Jun;4(2):173-7. doi: 10.1038/jcbfm.1984.25.
Focal ischemia was induced in 23 cats by occluding the left middle cerebral artery for 2 h. The animals were then divided into groups for unforced reperfusion of variable duration ranging from 2 to 48 h. Neurological ratings were obtained during both ischemia and reperfusion. Following planned sacrifice the regional ATP content was assessed by means of a bioluminescence method showing spatial distribution and degree of ATP depletion. All the animals developed a neurologic deficit, with a median of 6 points on a disability scale of 0-10. After reopening of the middle cerebral artery, neurologic recovery was quite variable depending on the initial neurologic deficit (partial phi = 0.67, p1 less than 0.05): Animals with mild initial functional impairment improved and those with severe neurologic disturbances either died early or developed a more severe neurologic deficit, irrespective of the duration of reperfusion. The degree of ATP depletion and the amount of brain tissue involved exhibited a significant correlation with the neurological outcome (tau = 0.50, p1 less than 0.05), but they were even more closely related to the initial neurologic deficit (partial phi = 1.00, p1 less than 0.001), suggesting an early definitive manifestation of deficiencies in regional energy metabolism.
通过阻断23只猫的左大脑中动脉2小时诱导局灶性缺血。然后将这些动物分为几组，进行2至48小时不等的非强迫性再灌注。在缺血和再灌注期间均进行神经功能评分。在计划处死动物后，通过生物发光法评估局部ATP含量，以显示ATP消耗的空间分布和程度。所有动物均出现神经功能缺损，在0至10的残疾量表上中位数为6分。大脑中动脉重新开放后，神经功能恢复情况差异很大，这取决于最初的神经功能缺损（偏相关系数phi = 0.67，p1小于0.05）：最初功能损害较轻的动物有所改善，而那些有严重神经功能障碍的动物要么早期死亡，要么出现更严重的神经功能缺损，与再灌注持续时间无关。ATP消耗程度和受累脑组织量与神经功能结局呈显著相关（tau = 0.50，p1小于0.05），但它们与最初的神经功能缺损关系更为密切（偏相关系数phi = 1.00，p1小于0.001），提示区域能量代谢缺陷的早期明确表现。
