Separation of sialyl-oligosaccharides by high-performance liquid chromatography. Application to analysis of carbohydrate units of acidic oligosaccharides obtained by hydrazinolysis of hen ovomucoid.

Sialyl- oligossacharides derived from hen ovomucoid by hydrazinolysis have been separated by liquid chromatography on quaternary amine bonded silica and alkylamine modified silicas . By using a mobile phase consisting of a mixture of acetonitrile and potassium dihydrogen phosphate with 0.01% of 1,4- diaminobutane , effective resolution of high-molecular-weight monosialylated oligossacharides was achieved in less than 2 h.