源自鸡卵类黏蛋白的新型N-糖苷碳水化合物单元的一级结构。一项500兆赫的氢核磁共振研究。
Primary structure of a novel N-glycosidic carbohydrate unit, derived from hen ovomucoid. A 500-MHz 1H-NMR study.
作者信息
Paz-Parente J, Strecker G, Leroy Y, Montreuil J, Fournet B, van Halbeek H, Dorland L, Vliegenthart J F
出版信息
FEBS Lett. 1983 Feb 21;152(2):145-52. doi: 10.1016/0014-5793(83)80367-6.
The N-glycosidic carbohydrate chains of hen beta-ovomucoid were released from the protein by hydrazinolysis, and separated by HPLC. Primary structural analysis of 3 major fractions was conducted by applying 500-MHz 1H-NMR spectroscopy in combination with methylation analysis. One of the fractions investigated appeared to consist of an intersected penta-antennary structure extended with one Gal residue. The location of the latter in a certain branch could be established unambiguously by NMR. This structure is a novel member of the family of N-glycosidic carbohydrates of glycoproteins.
通过肼解从蛋白质中释放出母鸡β-卵类粘蛋白的N-糖苷碳水化合物链，并通过高效液相色谱法进行分离。应用500兆赫兹的1H-NMR光谱结合甲基化分析对3个主要馏分进行一级结构分析。所研究的馏分之一似乎由一个带有一个半乳糖残基延伸的交叉五天线结构组成。后者在某个分支中的位置可以通过NMR明确确定。这种结构是糖蛋白N-糖苷碳水化合物家族的一个新成员。
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