采用电子捕获检测气相色谱法测定尿氯醛酸（三氯乙醇的葡萄糖醛酸共轭物）及其在尿液、血浆和肝脏中的应用。

Determination of urochloralic acid, the glucuronic acid conjugate of trichloroethanol, by gas chromatography with electron-capture detection and its application to urine, plasma and liver.

作者信息

Ikeda M, Hattori H, Koyama Y, Ohmori S

出版信息

J Chromatogr. 1984 Apr 13;307(1):111-9. doi: 10.1016/s0378-4347(00)84077-3.

Abstract

Gas chromatography with electron-capture detection was used to quantify the glucuronic acid conjugate of trichloroethanol, urochloralic acid. The conjugate was extracted into ethyl acetate--ethanol (19:1, v/v) under acidic conditions, and analyzed as its trifluoroacetylated methyl ester or as its acetylated methyl ester. As little as 0.2 microgram of the conjugate was quantified, and the method was applicable to urine, plasma and liver. The synthesis of urochloralic acid from trichloroethanol by rat liver microsomal fraction was demonstrated in the presence of uridine diphosphoglucuronic acid as coenzyme.

摘要

采用带电子捕获检测的气相色谱法对三氯乙醇的葡糖醛酸共轭物——尿氯醛酸进行定量分析。在酸性条件下，将该共轭物萃取到乙酸乙酯 - 乙醇（19:1，v/v）中，并以其三氟乙酰化甲酯或乙酰化甲酯形式进行分析。该共轭物的定量下限低至0.2微克，此方法适用于尿液、血浆和肝脏样本。在尿苷二磷酸葡糖醛酸作为辅酶存在的情况下，证实了大鼠肝脏微粒体部分可由三氯乙醇合成尿氯醛酸。