Kuriyama K, Ida S, Ohkuma S
J Neurochem. 1984 Jun;42(6):1600-6. doi: 10.1111/j.1471-4159.1984.tb12748.x.
Cerebral taurine biosynthesis in a spontaneously hypertensive rat (SHR) has been studied. Cysteine sulfinic acid (CSA) and cysteic acid (CA), possible key intermediates in taurine biosynthesis, were found in the rat brain, whereas no cysteamine-cystamine was detected. In the brain of SHR, a statistically significant decrease in the contents of CSA, CA, and taurine was noted in the cerebellum, hypothalamus, and striatum as compared with normotensive Wistar Kyoto rats. Similarly, it was demonstrated that the activity of cysteine dioxygenase, the enzyme catalyzing cysteine to CSA, was attenuated significantly in the same brain areas of SHR. In contrast, no alteration in the activity of CSA decarboxylase, the enzyme converting CSA to hypotaurine or CA to taurine, was observed. A decline in the percent conversion of [14C]cysteine to [14C]taurine was found also in tissue homogenates from the cerebellum, hypothalamus, and striatum of SHR, indicating that the declines in taurine content may be due to an attenuation of taurine biosynthesis, possibly at the step involving cysteine dioxygenase.
对自发性高血压大鼠（SHR）的脑内牛磺酸生物合成进行了研究。在大鼠脑中发现了半胱氨酸亚磺酸（CSA）和半胱氨酸磺酸（CA），它们可能是牛磺酸生物合成的关键中间体，而未检测到半胱胺 - 胱胺。与正常血压的Wistar Kyoto大鼠相比，在SHR的脑中，小脑、下丘脑和纹状体中CSA、CA和牛磺酸的含量有统计学意义的显著下降。同样，已证明在SHR的相同脑区中，催化半胱氨酸生成CSA的半胱氨酸双加氧酶的活性显著减弱。相比之下，未观察到将CSA转化为亚牛磺酸或将CA转化为牛磺酸的CSA脱羧酶的活性有改变。在SHR的小脑、下丘脑和纹状体的组织匀浆中也发现[14C]半胱氨酸向[14C]牛磺酸的转化率下降，这表明牛磺酸含量的下降可能是由于牛磺酸生物合成的减弱，可能是在涉及半胱氨酸双加氧酶的步骤中。
