Zhilina T N
Mikrobiologiia. 1978 May-Jun;47(3):396-9.
A pure culture of the obligate anaerobe methanosarcina, biotype 2, was isolated using an original technique of dilution in agar and inoculation into a preliminarily reduced medium. Additional organic substances, in particular yeast extract, are necessary for germination of a small number of packets in the course of isolation. The methanosarcina can use, during several inoculations, acetate as a sole source of carbon and energy, and does not require hydrogen as the main substrate of catabolism. The organic substances being tested (with an exception of pyruvate, hemin and cysteine which somewhat increased the lag phase) had no significant effect on growth and production of methane. Apparently, the mechanosarcina, biotype 2, should be referred to as an individual species.
采用琼脂稀释和接种到预先还原培养基中的原始技术，分离出了专性厌氧菌2型甲烷八叠球菌的纯培养物。在分离过程中，少量菌包的萌发需要额外的有机物质，特别是酵母提取物。在多次接种过程中，甲烷八叠球菌可以利用乙酸盐作为唯一的碳源和能源，并且不需要氢气作为分解代谢的主要底物。所测试的有机物质（除丙酮酸、血红素和半胱氨酸略有延长延迟期外）对生长和甲烷产生没有显著影响。显然，2型甲烷八叠球菌应被视为一个独立的物种。
