Mah R A, Smith M R, Baresi L
Appl Environ Microbiol. 1978 Jun;35(6):1174-84. doi: 10.1128/aem.35.6.1174-1184.1978.
An acetate-fermenting strain of Methanosarcina was isolated from an acetate enrichment culture inoculated with anaerobic sludge from a waste treatment digestor. In pure culture, this organism fermented acetate in the absence of added hydrogen at rates comparable in magnitude to those found in digestor systems. This rate was significantly higher than previously obtained for pure cultures of this genus. Mineral components of yeast extract were highly stimulatory for cultures growing on methanol. Comparable stimulation was not observed for cultures growing on acetate. Labeling studies indicated that acetate was converted to methane and CO2 as predicted by previous studies on mixed cultures. Total oxidation or reduction of acetate was not the mechanism of conversion of acetate to methane by the pure culture. The ability of this strain to form colonies or to produce methane from acetate was apparently influenced by the choice of substrate and conditions used for growing the inoculum.
从接种了来自废物处理消化器厌氧污泥的乙酸盐富集培养物中分离出一株产甲烷八叠球菌属的乙酸发酵菌株。在纯培养中，该微生物在不添加氢气的情况下发酵乙酸盐的速率与在消化器系统中发现的速率相当。该速率显著高于此前该属纯培养物所获得的速率。酵母提取物的矿物质成分对以甲醇为培养基生长的培养物具有高度刺激作用。对于以乙酸盐为培养基生长的培养物，未观察到类似的刺激作用。标记研究表明，乙酸盐如先前对混合培养物的研究所预测的那样转化为甲烷和二氧化碳。乙酸盐的完全氧化或还原不是该纯培养物将乙酸盐转化为甲烷的机制。该菌株形成菌落或从乙酸盐产生甲烷的能力显然受到用于培养接种物的底物和条件选择的影响。
