De Simone C, Ferrari M, Pugnaloni L, Ferrarelli G, Rumi C, Sorice F
Immunol Lett. 1986 Jan;12(1):37-41. doi: 10.1016/0165-2478(86)90078-7.
The aim of the present work was to develop an in vitro model of eosinophil cytotoxicity which mimics numerous in vivo situations characterized by complement activation through the alternative pathway. Our results demonstrate that eosinophilic granulocytes from patients with parasitic diseases and blood eosinophilia were able to damage chicken red blood cells when incubated with zymosan-activated serum (ZAS) as assessed by a 51Cr release assay. The phenomenon was independent of the presence of antibodies directed against the target cells and related to the quantity of ZAS added to the wells. As target cell lysis is totally or partially inhibited by catalase, sodium azide and potassium cyanide, an involvement of toxic oxygen derivatives as cytolytic mediators was suggested.
本研究的目的是建立一种嗜酸性粒细胞细胞毒性的体外模型，该模型模拟了许多体内通过替代途径激活补体的情况。我们的结果表明，通过51Cr释放试验评估，寄生虫病患者和血液嗜酸性粒细胞增多患者的嗜酸性粒细胞在与酵母聚糖激活血清（ZAS）孵育时能够损伤鸡红细胞。该现象与针对靶细胞的抗体的存在无关，并且与添加到孔中的ZAS的量有关。由于过氧化氢酶、叠氮化钠和氰化钾完全或部分抑制了靶细胞的裂解，提示有毒氧衍生物作为细胞溶解介质参与其中。
