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基孔肯雅病毒的结构蛋白。

Structural proteins of Chikungunya virus.

作者信息

Simizu B, Yamamoto K, Hashimoto K, Ogata T

出版信息

J Virol. 1984 Jul;51(1):254-8. doi: 10.1128/JVI.51.1.254-258.1984.

DOI:10.1128/JVI.51.1.254-258.1984
PMID:6726893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254427/
Abstract

Polyacrylamide gel analysis of the structural proteins of African and Asian strains of Chikungunya virus, an alphavirus, showed that both strains contain three structural proteins: glycosylated E1 and E2, embedded in the viral envelope, and a nonglycosylated nucleocapsid protein. In pulse-chase experiments the precursor protein PE2 was chased into glycoprotein E2, which migrated slightly faster than did glycoprotein E1. The third Chikungunya glycoprotein, E3, was not associated with mature virions but was released into culture fluids. With glycoproteins E1 and E2, separated by glass wool column chromatography, it was shown that hemagglutinating activity is associated with glycoprotein E1.

摘要

对甲病毒基孔肯雅病毒非洲株和亚洲株的结构蛋白进行聚丙烯酰胺凝胶分析表明，这两种毒株均含有三种结构蛋白：嵌入病毒包膜中的糖基化E1和E2，以及一种非糖基化核衣壳蛋白。在脉冲追踪实验中，前体蛋白PE2转变为糖蛋白E2，其迁移速度略快于糖蛋白E1。基孔肯雅病毒的第三种糖蛋白E3不与成熟病毒粒子相关，而是释放到培养液中。通过玻璃棉柱色谱法分离糖蛋白E1和E2后发现，血凝活性与糖蛋白E1相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/52e05619f2c8/jvirol00130-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/084690343fcb/jvirol00130-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/46e73be1a340/jvirol00130-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/d2b0346755fc/jvirol00130-0264-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/4ca0d28ef6bb/jvirol00130-0264-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/52e05619f2c8/jvirol00130-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/084690343fcb/jvirol00130-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/46e73be1a340/jvirol00130-0264-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/d2b0346755fc/jvirol00130-0264-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/4ca0d28ef6bb/jvirol00130-0264-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a60b/254427/52e05619f2c8/jvirol00130-0265-a.jpg

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Purification of the envelope glycoproteins of western equine encephalitis virus by glass wool column chromatography.
巴西人群传播本地流行的基孔肯雅病毒谱系的高能力。
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High capacity of Brazilian populations to transmit a locally circulating lineage of Chikungunya virus.巴西人群传播本地流行的基孔肯雅病毒谱系的能力很强。
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An Overview of Indian Biomedical Research on the Chikungunya Virus with Particular Reference to Its Vaccine, an Unmet Medical Need.印度关于基孔肯雅病毒的生物医学研究概述，特别提及针对该病毒的疫苗，这是一项未满足的医疗需求。
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