pH 对染色质核心颗粒稳定性的影响。
Effects of pH on the stability of chromatin core particles.
作者信息
Libertini L J, Small E W
出版信息
Nucleic Acids Res. 1984 May 25;12(10):4351-9. doi: 10.1093/nar/12.10.4351.
Abstract
Chromatin core particles near physiological ionic strength undergo a reversible transition induced by changes in pH near neutrality. While sedimentation studies indicate no significant effect on size or shape, changes in tyrosine fluorescence anisotropy and in circular dichroism suggest a somewhat looser structure at high pH. Further support of this suggestion is given by high salt dissociation experiments; at pH 8 core particles begin to show changes at lower salt concentration than at pH 6. The pH transition appears unaffected by the presence of Mg2+ but can be blocked by crosslinking of the histones. A possible relationship is suggested between this transition and increases in intracellular pH which correlate with enhancement in several aspects of cellular activity including DNA replication.
摘要
在接近生理离子强度的条件下，染色质核心颗粒会因接近中性的pH值变化而发生可逆转变。虽然沉降研究表明对颗粒大小和形状没有显著影响，但酪氨酸荧光各向异性和圆二色性的变化表明，在高pH值下结构略显松散。高盐解离实验进一步支持了这一观点；在pH 8时，核心颗粒在比pH 6更低的盐浓度下就开始出现变化。pH转变似乎不受Mg2+存在的影响，但可被组蛋白的交联所阻断。这种转变与细胞内pH值升高之间可能存在某种关系，细胞内pH值升高与包括DNA复制在内的细胞活动多个方面的增强相关。
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本文引用的文献
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Structural changes of soluble rat liver chromatin induced by the shift in pH from 7 to 9.pH从7转变为9所诱导的可溶性大鼠肝脏染色质的结构变化。
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Biochemistry. 1982 Jul 6;21(14):3327-34. doi: 10.1021/bi00257a013.
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