Thomas J O, Kornberg R D
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626-30. doi: 10.1073/pnas.72.7.2626.
Crosslinking with dimethyl suberimidate reveals a chain of histone octamers in chromatin. The octamer can be isolated free in solution at high ionic strength and pH. The identification of dimers formed by crosslinking reveals two or more contacts of each histone with others within the octamer. The molecular weight (110,000) and pattern of dissociation of the octamer are compatible with the composition (F2A1)2(F3)2(F2A2)2(F2B)2.
与亚辛二酸二甲酯交联揭示了染色质中组蛋白八聚体链。八聚体可以在高离子强度和pH值下在溶液中自由分离。通过交联形成的二聚体的鉴定揭示了每个组蛋白与八聚体内其他组蛋白的两个或更多个接触点。八聚体的分子量（110,000）和解离模式与组成（F2A1）2（F3）2（F2A2）2（F2B）2相符。
