Duplaix M, Sexton T J
Poult Sci. 1984 Apr;63(4):775-80. doi: 10.3382/ps.0630775.
Two experiments were conducted to determine the relationship between thawing temperature and the type of straw in which chicken semen was frozen. In Experiment 1, semen was frozen in three different types of plastic straws: US (.5-ml capacity), French (.5-ml capacity), and French mini (.25-ml capacity). Experiment 1 was divided into two trials to compare semen packaged in the different straws and thawed at 15 (Trial 1) or .5 C (Trial 2). Although there were distinguishable features of the freeze and thaw curves between samples frozen in the different straws, the type of freeze straw had no effect on the fertilizing capacity of frozen semen when thaw temperature was held constant: fertility, Days 2 to 4 after artificial insemination, ranged from 16 to 27% for Trial 1 and 45 to 47% for Trial 2. In Experiment 2, semen was frozen in US straws and thawed at either .5 or 15 C to assess the effect of the thaw temperature. Fertility of frozen semen, Days 2 to 4 after artificial insemination, was significantly higher when semen was thawed at .5 than at 15 C (62 vs. 20%).
进行了两项实验以确定解冻温度与冷冻鸡精液所用细管类型之间的关系。在实验1中，精液被冷冻在三种不同类型的塑料细管中：美国细管（容量0.5毫升）、法国细管（容量0.5毫升）和法国微型细管（容量0.25毫升）。实验1分为两个试验，以比较包装在不同细管中并在15℃（试验1）或0.5℃（试验2）解冻的精液。尽管在不同细管中冷冻的样本之间的冻融曲线有明显特征，但当解冻温度保持恒定时，冷冻细管的类型对冷冻精液的受精能力没有影响：人工授精后第2至4天的受精率，试验1为16%至27%，试验2为45%至47%。在实验2中，精液被冷冻在美国细管中，并在0.5℃或15℃解冻，以评估解冻温度的影响。人工授精后第2至4天，冷冻精液在0.5℃解冻时的受精率显著高于在15℃解冻时（62%对20%）。
