Haak H L, Goselink H M, Veenhof W F, Blotkamp J, Jansen J
Scand J Haematol. 1984 May;32(5):515-24. doi: 10.1111/j.1600-0609.1984.tb02194.x.
The proliferation of human bone marrow in methylcellulose stimulated by various concentrations of conditioned medium (CM) and observed at various intervals was studied. The growth kinetics of granulocytic aggregates was found to differ from monocytic clusters and colonies. Granulocytic aggregates showed a consistent and reproducible dose-response relationship; at day 7, the maximum number of granulocytic aggregates was found at 4% CM. At higher levels, the total number of aggregates decreased, while the number of cells per aggregate increased. The number of macrophage aggregates was far less, depending on the CM concentration. Photographic interval studies showed that at high concentrations of CM, clusters and colonies were formed earlier, but also coalesce to form one colony. Our results suggest that the proliferation kinetics of granulocytic aggregates are complex and preclude simple statements about sensitivity to colony-stimulating activity.
研究了在不同浓度条件培养基（CM）刺激下，人骨髓在甲基纤维素中的增殖情况，并在不同时间间隔进行观察。发现粒细胞聚集体的生长动力学与单核细胞簇和集落不同。粒细胞聚集体呈现出一致且可重复的剂量反应关系；在第7天，4%的CM时粒细胞聚集体数量最多。浓度更高时，聚集体总数减少，而每个聚集体中的细胞数量增加。巨噬细胞聚集体的数量少得多，这取决于CM浓度。摄影间隔研究表明，在高浓度CM下，簇和集落形成得更早，但也会合并形成一个集落。我们的结果表明，粒细胞聚集体的增殖动力学很复杂，难以简单说明其对集落刺激活性的敏感性。
