给动物施用氟化皮质类固醇后，在二苯乙烯衍生物的放射免疫测定中出现假阳性结果。
False-positive results in the radioimmunoassay detection of stilbene derivatives after administration of fluorinated corticosteroids to animals.
作者信息
Brunn H, Fister P, Eigenbrodt E, Stojanowić V
出版信息
Food Chem Toxicol. 1985 Oct;23(10):931-5. doi: 10.1016/0278-6915(85)90110-3.
During routine inspections, indications were found that the therapeutic use of fluocortolone or dexamethasone can cause false-positive results in the detection of stilbene derivatives by radioimmunoassay (RIA) in samples of animal origin. After experimental application of the fluorinated corticosteroids to rabbits or calves, the assumed false-positive RIA results were confirmed. The kinetics after application of dexamethasone were concomitant with the elimination of radioactive [3H]dexamethasone. Furthermore, it could be demonstrated that [3H]dexamethasone was transformed in the faeces of rabbits and calves. The metabolites formed were not completely separated by the extraction procedure used for the RIA and seemed to be responsible for the false-positive results.
在常规检查中发现，氟轻松或地塞米松的治疗性使用会在动物源性样本的放射免疫分析（RIA）中导致二苯乙烯衍生物检测出现假阳性结果。在对兔子或小牛进行氟化皮质类固醇的实验性应用后，证实了假定的RIA假阳性结果。地塞米松应用后的动力学与放射性[3H]地塞米松的消除情况一致。此外，可以证明[3H]地塞米松在兔子和小牛的粪便中发生了转化。所形成的代谢产物未通过用于RIA的提取程序完全分离，似乎是导致假阳性结果的原因。
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