Naegleria: observations on its distribution in east Asia and South America, and electron microscopic studies on cultured trophozoites.

The present study is a survey of the distribution of Naegleria strains in water collected from ponds and rivers. Twenty three strains were isolated and identified as Naegleria fowleri. These strains were examined in culture for temperature tolerance. The five strains among of them were able to survive 32-36 degrees C. The two virulent strains were isolated from above the five strains. The lamellar structures in Naegleria showed positive reaction for acid phosphatase activity.