Sunderman F W, Crisostomo M C, Reid M C, Hopfer S M, Nomoto S
Ann Clin Lab Sci. 1984 May-Jun;14(3):232-41.
A method is described for analysis of nickel in serum and heparinized blood. After the sample has been subjected to protein precipitation with nitric acid and heat, nickel in the protein-free extract is measured by electrothermal atomic absorption with Zeeman background correction. The method is more sensitive, rapid, and convenient than previous techniques, and less subject to nickel contamination. Nickel concentrations in serums from New Zealand rabbits (mean +/- SD) average 4.0 +/- 2.5 micrograms per L, range = 0.9 to 11.9, N = 30; these results agree with measurements by the International Union of Pure and Applied Chemistry (IUPAC) reference procedure ( corr . coef . = 0.98). The following values are reported for nickel concentrations in serum and whole blood from healthy adult persons living in central Connecticut: serum, 0.46 +/- 0.26 micrograms per L, range = 0.1 to 1.3, N = 39; whole blood, 1.26 +/- 0.33 micrograms per L, range = 0.6 to 1.8, N = 30. This method is suitable for routine use in clinical and industrial laboratories.
本文描述了一种分析血清和肝素化血液中镍的方法。样品经硝酸和加热进行蛋白质沉淀后，采用塞曼背景校正的电热原子吸收法测定无蛋白提取物中的镍。该方法比以前的技术更灵敏、快速、方便，且受镍污染的影响更小。新西兰兔血清中的镍浓度（平均值±标准差）平均为每升4.0±2.5微克，范围为0.9至11.9，N = 30；这些结果与国际纯粹与应用化学联合会（IUPAC）参考程序的测量结果一致（相关系数= 0.98）。以下是居住在康涅狄格州中部的健康成年人血清和全血中镍浓度的报告值：血清，每升0.46±0.26微克，范围为0.1至1.3，N = 39；全血，每升1.26±0.33微克，范围为0.6至1.8，N = 30。该方法适用于临床和工业实验室的常规使用。
