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关于外淋巴(豚鼠)生化研究中的误差来源

On sources of error in the biochemical study of perilymph (guinea-pig).

作者信息

Scheibe F, Haupt H, Bergmann K

出版信息

Arch Otorhinolaryngol. 1984;240(1):43-8. doi: 10.1007/BF00464343.

DOI:10.1007/BF00464343
PMID:6732610
Abstract

Contamination of perilymph with other fluids (cerebrospinal fluid, tissue fluid, blood, endolymph) together with sampling, anaesthesia, surgical intervention or food intake of the animals may considerably affect the analytical result. The numerous possible artefacts seem to be the main reason why varying values are given in the literature for the same chemical component of perilymph. This is also partly true of cerebrospinal fluid and blood. The effect of some sources of error on selected chemical components of perilymph, cerebrospinal fluid and blood is briefly summarized.

摘要

外淋巴液被其他液体(脑脊液、组织液、血液、内淋巴液)污染,再加上动物采样、麻醉、外科手术干预或食物摄入,可能会极大地影响分析结果。众多可能的假象似乎是文献中关于外淋巴液同一化学成分给出不同值的主要原因。脑脊液和血液也部分存在这种情况。本文简要总结了某些误差来源对外淋巴液、脑脊液和血液特定化学成分的影响。

相似文献

1
On sources of error in the biochemical study of perilymph (guinea-pig).关于外淋巴(豚鼠)生化研究中的误差来源
Arch Otorhinolaryngol. 1984;240(1):43-8. doi: 10.1007/BF00464343.
2
Biochemical differences between perilymph, cerebrospinal fluid and blood plasma in the guinea pig.豚鼠外淋巴、脑脊液和血浆之间的生化差异。
Hear Res. 1985 Jan;17(1):61-6. doi: 10.1016/0378-5955(85)90131-5.
3
[Lactate and pyruvate concentrations in perilymph, blood, and cerebrospinal fluid of guinea pigs].[豚鼠外淋巴、血液和脑脊液中乳酸和丙酮酸的浓度]
Arch Otorhinolaryngol. 1981;232(1):81-9. doi: 10.1007/BF00661006.
4
[Biochemical analysis of the endolymph and perilymph of the trout (Salmo gairdneri) (author's transl)].虹鳟(Salmo gairdneri)内淋巴和外淋巴的生化分析(作者译)
Laryngol Rhinol Otol (Stuttg). 1982 Jan;61(1):12-6.
5
Chemical composition in various compartments of inner ear fluid.内耳液各腔室中的化学成分。
Arch Otorhinolaryngol. 1978 Jul 26;220(4):259-64. doi: 10.1007/BF00455361.
6
Amino acid profiles in inner ear fluids and cerebrospinal fluid.内耳液和脑脊液中的氨基酸谱。
Laryngoscope. 1982 Mar;92(3):321-8. doi: 10.1288/00005537-198203000-00018.
7
[Protein concentration in the guinea-pig perilymph].[豚鼠外淋巴中的蛋白质浓度]
Acta Otolaryngol. 1975 Jan-Feb;79(1-2):51-7. doi: 10.3109/00016487509124654.
8
Biochemical parameters of guinea pig perilymph sampled according to scala and following sound presentation.根据蜗管采样并在声音呈现后采集的豚鼠外淋巴的生化参数。
Environ Health Perspect. 1974 Aug;8:157-64. doi: 10.1289/ehp.748157.
9
Species differences in inner ear fluids.内耳液中的物种差异。
Arch Otorhinolaryngol. 1980;228(3):187-93. doi: 10.1007/BF00454226.
10
The effect of furosemide on sodium and potassium concentrations in guinea-pig perilymph.呋塞米对豚鼠外淋巴中钠和钾浓度的影响。
Arch Otorhinolaryngol. 1984;240(1):21-6. doi: 10.1007/BF00464340.

引用本文的文献

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Hearing loss in hydrocephalus: a review, with focus on mechanisms.脑积水所致听力损失:综述,重点关注机制
Neurosurg Rev. 2016 Jan;39(1):13-24; discussion 25. doi: 10.1007/s10143-015-0650-2. Epub 2015 Aug 18.
2
Therapy of hearing disorders - conservative procedures.听力障碍的治疗——保守治疗方法。
GMS Curr Top Otorhinolaryngol Head Neck Surg. 2005;4:Doc01. Epub 2005 Sep 28.
3
Pharmacokinetics of Drug Entry into Cochlear Fluids.药物进入耳蜗液的药代动力学。

本文引用的文献

1
[Lactate and pyruvate concentrations in perilymph, blood, and cerebrospinal fluid of guinea pigs].[豚鼠外淋巴、血液和脑脊液中乳酸和丙酮酸的浓度]
Arch Otorhinolaryngol. 1981;232(1):81-9. doi: 10.1007/BF00661006.
2
A scanning electron microscopic study of the guinea pig cochlear aqueduct. With emphasis on fracture preparation.豚鼠耳蜗导水管的扫描电子显微镜研究。重点在于骨折制备。
ORL J Otorhinolaryngol Relat Spec. 1981;43(2):79-88. doi: 10.1159/000275529.
3
[Microflame-photometric estimation of sodium and potassium for testing the purity of inner ear fluids (author's transl)].
Volta Rev. 2005;105(3):277-298.
4
Demonstration of a longitudinal concentration gradient along scala tympani by sequential sampling of perilymph from the cochlear apex.通过对耳蜗顶端外淋巴进行连续采样来证明沿鼓阶的纵向浓度梯度。
J Assoc Res Otolaryngol. 2006 Jun;7(2):182-93. doi: 10.1007/s10162-006-0034-y. Epub 2006 Apr 22.
5
Cochlear microdialysis for quantification of dexamethasone and fluorescein entry into scala tympani during round window administration.圆窗给药期间,采用耳蜗微透析法对鼓阶内的地塞米松和荧光素进入量进行定量分析。
Hear Res. 2006 Feb;212(1-2):236-44. doi: 10.1016/j.heares.2005.12.001. Epub 2006 Jan 25.
6
Perilymph sampling from the cochlear apex: a reliable method to obtain higher purity perilymph samples from scala tympani.从耳蜗顶部采集外淋巴:一种从鼓阶获取更高纯度外淋巴样本的可靠方法。
J Neurosci Methods. 2006 May 15;153(1):121-9. doi: 10.1016/j.jneumeth.2005.10.008. Epub 2005 Nov 28.
7
Local inner-ear drug delivery and pharmacokinetics.局部内耳给药与药代动力学。
Drug Discov Today. 2005 Oct 1;10(19):1299-306. doi: 10.1016/S1359-6446(05)03574-9.
8
The cochlear aqueduct in pediatric temporal bones.小儿颞骨中的蜗水管。
Eur Arch Otorhinolaryngol. 1997;254 Suppl 1:S34-8. doi: 10.1007/BF02439718.
用于检测内耳液纯度的钠和钾的微火焰光度测定法(作者译)
Arch Otorhinolaryngol. 1982;234(1):45-51. doi: 10.1007/BF00453537.
4
Amino acid profiles in inner ear fluids and cerebrospinal fluid.内耳液和脑脊液中的氨基酸谱。
Laryngoscope. 1982 Mar;92(3):321-8. doi: 10.1288/00005537-198203000-00018.
5
[On the glucose, pyruvate, and lactate concentration of perilymph, blood, and cerebrospinal fluid of unexposed and sound-exposed guinea pigs under ethyl urethane anesthesia (author's transl)].[在氨基甲酸乙酯麻醉下未暴露和暴露于声音的豚鼠的外淋巴、血液和脑脊液中的葡萄糖、丙酮酸和乳酸浓度(作者译)]
Arch Otorhinolaryngol. 1981;233(1):89-97. doi: 10.1007/BF00464278.
6
[Postmortem changes in the perilymphatic lactate and pyruvate concentrations of guinea pigs. (author's transl)].豚鼠外淋巴液中乳酸和丙酮酸浓度的死后变化。(作者译)
Arch Otorhinolaryngol. 1981;232(3):293-7. doi: 10.1007/BF00457455.
7
[Total lactate dehydrogenase activity of perilymph, plasma and cerebrospinal fluid in unstressed and noise stressed guinea pigs].[未受应激和噪声应激豚鼠外淋巴、血浆和脑脊液中乳酸脱氢酶的总活性]
Arch Otorhinolaryngol. 1983;238(1):77-85. doi: 10.1007/BF00453744.
8
Amino acid content of guinea pig perilymph collected under conditions of quiet or sound stimulation.在安静或声音刺激条件下收集的豚鼠外淋巴液中的氨基酸含量。
Hear Res. 1982 May;7(1):13-8. doi: 10.1016/0378-5955(82)90079-x.
9
[Experimental studies on places of formation and metabolism of perilymph].[外淋巴形成部位及代谢的实验研究]
Acta Otolaryngol. 1966:Suppl 212:1+.
10
Perilymph in patients with otosclerosis. Comparisons with capillary serum, venous serum, and cerebrospinal fluid.耳硬化症患者的外淋巴。与毛细血管血清、静脉血清及脑脊液的比较。
Arch Otolaryngol. 1966 Oct;84(4):373-94. doi: 10.1001/archotol.1966.00760030375002.