Hashimoto S, Morton R E, Zilversmit D B
Biochem Biophys Res Commun. 1984 Apr 30;120(2):586-92. doi: 10.1016/0006-291x(84)91295-6.
The accessibility of intracellular membrane cholesteryl esters to removal was tested with plasma lipid transfer protein as a tool. Incubation of a mixture of non-radioactive smooth microsomes + rough microsomes prelabeled with cholesteryl ester resulted in slight movement (2-4%) of radioactive cholesteryl ester into smooth microsomes. With the addition of increasing amounts of plasma lipid transfer protein to the mixture, the % transfer of cholesteryl ester into smooth microsomes progressively increased until a plateau was reached at 14%. Movement of cholesteryl ester in the reverse direction was examined with non-radioactive rough microsomes as an acceptor and smooth microsomes prelabeled with cholesteryl ester as a donor. The pattern of the % cholesteryl ester transferred in the reverse and forward direction was almost identical in the presence of plasma lipid transfer protein, showing bidirectional movement of cholesteryl ester between membranes.
以血浆脂质转运蛋白作为工具，测试细胞内膜胆固醇酯的去除可及性。将预先用胆固醇酯标记的非放射性滑面微粒体与糙面微粒体的混合物进行孵育，导致放射性胆固醇酯有轻微移动（2%-4%）进入滑面微粒体。随着向混合物中添加越来越多的血浆脂质转运蛋白，胆固醇酯向滑面微粒体的转移百分比逐渐增加，直至达到14%的平台期。以非放射性糙面微粒体作为受体，预先用胆固醇酯标记的滑面微粒体作为供体，检测胆固醇酯的反向移动。在血浆脂质转运蛋白存在的情况下，胆固醇酯在反向和正向转移的百分比模式几乎相同，表明胆固醇酯在膜之间双向移动。
