Wetterau J R, Zilversmit D B
Biochim Biophys Acta. 1986 Feb 28;875(3):610-7. doi: 10.1016/0005-2760(86)90084-6.
A triacylglycerol and cholesteryl ester transfer activity has been isolated from rat liver. After homogenization, the liver cells were subfractionated into the 10 000 X g pellet, microsomal fraction and postmicrosomal supernatant. Most of the transfer activity appeared to be associated with the microsomal fraction. Rough and smooth microsomes contained nearly equal transfer activities. When isolated microsomes were subject to proteolytic attack, the transfer activity was not inactivated, unless it had been released from the microsomes prior to proteolytic treatment. This indicates that the activity is probably located within the microsomal vesicles. Similar transfer activities were found in the intestinal mucosa of rats, whereas little or no activity was detected in the brain, heart, kidney, or plasma.
已从大鼠肝脏中分离出一种三酰甘油和胆固醇酯转移活性物质。匀浆后，肝细胞被亚分级分离为10000×g沉淀、微粒体部分和微粒体后上清液。大部分转移活性似乎与微粒体部分相关。粗面微粒体和滑面微粒体含有几乎相等的转移活性。当分离的微粒体受到蛋白水解攻击时，转移活性不会失活，除非它在蛋白水解处理之前已从微粒体中释放出来。这表明该活性可能位于微粒体囊泡内。在大鼠的肠黏膜中发现了类似的转移活性，而在脑、心脏、肾脏或血浆中几乎未检测到活性。
