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Ultraviolet-crosslinking reveals specific affinity of eukaryotic initiation factors for Semliki Forest virus mRNA.

作者信息

Setyono B, Van Steeg H, Voorma H O

出版信息

Biochim Biophys Acta. 1984 Jul 18;782(3):242-6. doi: 10.1016/0167-4781(84)90058-7.

DOI:10.1016/0167-4781(84)90058-7
PMID:6733107
Abstract

Eukaryotic initiation factors (eIF) associate readily with 32P-labeled Semliki Forest virus (SFV) mRNA in vitro, forming complexes which can be crosslinked by 254 nm ultraviolet irradiation. After ribonuclease digestion, the initiation factors were released and analysed by gel electrophoresis. Autoradiography revealed proteins by virtue of crosslinked 32P-labeled mRNA fragments. eIF-4A, -4B and -4C as well as three subunits of eIF-3 could be crosslinked with SFV mRNA. None of these proteins bound to ribosomal RNAs.

摘要

相似文献

1
Ultraviolet-crosslinking reveals specific affinity of eukaryotic initiation factors for Semliki Forest virus mRNA.
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2
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Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins S1, S3/3a, S6 and S11 within the 48S pre-initiation complex.在48S起始前复合物中，信使核糖核酸（mRNA）与起始因子eIF-3、24 kDa帽结合蛋白以及核糖体蛋白S1、S3/3a、S6和S11发生交联。
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