Diatlovitskaia E V, Petkova D Kh, Lemenovskaia A F, Bergel'son L D
Biokhimiia. 1984 Apr;49(4):551-5.
The effects of the phospholipid composition of rat liver microsomes on the differential binding spectra of cytochrome P-450 with type I and II substrates (hexobarbital and aniline) were studied. Lysophosphatidylcholine was found to decrease the binding of both type substrates. These changes were reversible, and the spectra were restored by exchanging the microsomal lysophosphatidylcholine for unsaturated phosphatidylcholines, but not with dipalmitoylglycerophosphocholine, sphingomyelin, phosphatidylserine, and phosphatidylethanolamine. Phosphatidylinositol favoured only the binding of type II substrates. The data obtained indicate that the substrate binding capacity of cytochrome P-450 depends specifically on the "boundary" lipids, but not on the bulk lipids of the microsomal membrane.
研究了大鼠肝脏微粒体磷脂组成对细胞色素P - 450与I型和II型底物（己巴比妥和苯胺）差异结合光谱的影响。发现溶血磷脂酰胆碱会降低两种类型底物的结合。这些变化是可逆的，通过将微粒体溶血磷脂酰胆碱换成不饱和磷脂酰胆碱可恢复光谱，但用二棕榈酰甘油磷酸胆碱、鞘磷脂、磷脂酰丝氨酸和磷脂酰乙醇胺则不能。磷脂酰肌醇仅有利于II型底物的结合。所获得的数据表明，细胞色素P - 450的底物结合能力特别取决于“边界”脂质，而不是微粒体膜的主体脂质。
