Spang-Thomsen M, Vindeløv L L
Cell Tissue Kinet. 1984 Jul;17(4):401-10. doi: 10.1111/j.1365-2184.1984.tb00599.x.
The technique of labelled mitoses and flow cytometric DNA analysis were used to determine the proliferation kinetics of a human malignant melanoma grown in nude mice. The effect of tumour volume and of long-term serial transplantation on the kinetic parameters was investigated. The results showed that the cell loss factor, which was the dominant factor in the growth of this melanoma, increased from 52 to 69% with increasing tumour size, whereas the calculated growth fraction showed no systematic changes. The cell generation time increased from 34 to 44 hr with tumour size, mainly due to a prolongation of the G1 duration time, whereas no significant changes occurred in the duration of the S and G2 phases of the cell cycle. The stability of the investigated tumour characteristics indicated that the kinetics of this melanoma remained unchanged during more than sixty serial transplantations in nude mice. The methods applied are suitable for a detailed description of tumour growth kinetics, since they provide complementary results.
运用标记有丝分裂技术和流式细胞术DNA分析来测定在裸鼠体内生长的人恶性黑色素瘤的增殖动力学。研究了肿瘤体积和长期连续传代对动力学参数的影响。结果显示，细胞丢失因子是该黑色素瘤生长的主导因素，随着肿瘤大小增加，其从52%增至69%，而计算得出的生长分数未呈现系统性变化。细胞生成时间随肿瘤大小从34小时增加至44小时，主要是由于G1期持续时间延长，而细胞周期的S期和G2期持续时间未发生显著变化。所研究的肿瘤特征的稳定性表明，在裸鼠体内进行六十多次连续传代期间，该黑色素瘤的动力学保持不变。所应用的方法适用于详细描述肿瘤生长动力学，因为它们提供了互补的结果。
