Leclerc C, Bahr G M, Chedid L
Cell Immunol. 1984 Jul;86(2):269-77. doi: 10.1016/0008-8749(84)90381-2.
Activation of peritoneal exudate macrophages of mice to inhibit the in vitro proliferation of tumor target cells was achieved with low concentrations of N-acetyl-L-alanyl-D-isoglutamine (MDP for muramyl dipeptide) conjugated to a synthetic carrier. Addition to the cultures of monoclonal anti-MDP or anti-carrier antibodies renders a thousandfold-smaller concentration of the conjugate highly effective in activating macrophages. This synergistic effect was observed neither with a control monoclonal antibody of different specificity nor with an F(ab)2 fragment of the monoclonal anti-MDP antibody. Other controls, such as addition to the cultures of the carrier alone with its specific monoclonal antibodies, also demonstrated that there exists a requirement for the presence of MDP in the conjugate. The possible uses of such a system as well as the underlying mechanisms are discussed.
通过将低浓度的与合成载体偶联的N-乙酰-L-丙氨酰-D-异谷氨酰胺（MDP，即胞壁酰二肽）作用于小鼠腹膜渗出巨噬细胞，可实现对肿瘤靶细胞体外增殖的抑制。向培养物中添加单克隆抗MDP或抗载体抗体，可使浓度千分之一的偶联物在激活巨噬细胞方面高度有效。在不同特异性的对照单克隆抗体或单克隆抗MDP抗体的F(ab)2片段中均未观察到这种协同效应。其他对照，如向培养物中单独添加载体及其特异性单克隆抗体，也表明偶联物中存在MDP是必需的。本文讨论了该系统的可能用途及其潜在机制。
